ISOLAMENTO E AVALIAÇÃO DE BACTÉRIAS DO GÊNERO BACILLUS COM POTENCIAL PARA USO EM BIOCIMENTAÇÃO NA PRODUÇÃO DE ARGAMASSAS
DOI:
https://doi.org/10.46421/entac.v17i1.1300Palavras-chave:
Bacillus, Biomortar, BiocementationResumo
This study had as aim to isolate bacteria of the genus Bacillus from soil samples, in order to carry out an investigation of its potential in the production of urease, so that it can be used in biocementation. From a clayish latossoil in the horizont B, a soil sample was extracted, diluted in saline solution and put through a temperature of 80ºC in order to separate only colonies of bacteria that produce urease. From the solution, a solid biomass was produced in which the colonies got sporulated. After that, the colonies were revitalized so they could be used in the manufacture of mortar. A pilot test was made utilizing the bacterias instead of cement in the mortar and after 28 days the specimen was tested in its compression and traction. The results did not reach the minimum of resistance established by the technical rules but pozzolanic activity could be observed because the sample with no bacterial use was 50% less resistent than the rest. Therefore, it is possible to replace other cement materials for urease production bacteria.
Referências
ABO-EL-ENEIN, S. A. et al. Application of microbial biocementation to improve the physico-mechanical properties of cement mortar. HBRC Journal, Cairo, v. 9, n. 1, p.
36-40, 12 February 2012. ISSN 1687-4048. Disponivel em:<http://www.sciencedirect.com/science/article/pii/S1687404812000260?via%3Dihub#!>. Acesso em: 17 mar. 2018. Doi: https://doi.org/10.1016/j.hbrcj.2012.10.004.
ALSHALIF, A., F. et al. Carbon Dioxide (CO2) Sequestration In BioConcrete, An Overview, MATEC Web of Conferences, n. 103, 2016. Disponível em:<https://www.matecconferences.org/articles/matecconf/pdf/2017/17/matecconf_iscee2017_05016.pdf>Acesso em: 22 mar. 2018. Doi: 10.1051/matecconf/20171030BALAM, N. H.; MOSTOFINEJAD, D.; EFTEKHAR, M. Use of carbonate precipitating bacteria to reduce water absorption of aggregates, Construction and Building Materials, v. 141, p. 565-577, October 2017. ISSN 0950-0618 Disponivel em: <https://www.researchgate.net/profile/Nafise_Balam/publication/315177272_Use_of_carbonate_precipitating_bacteria_to_reduce_water_absorption_of_aggregates/links/59e700b3a6fdcc0e882d4649/Use-of-carbonate-precipitating-bacteria-to-reducewater-absorption-of-aggregates.pdf>.
Acesso em: 18 mar. 2018. Doi: https://doi.org/10.1016/j.conbuildmat.2017.03.042.
IDEIES INSTITUTO DE DESENVOLVIMENTO EDUCACIONAL E INDUSTRIAL DO ESPIRITO SANTO. Análise de Competitividade do Setor das Indústrias de Argamassa do Estado do Espírito Santo, Vitória, Espírito Santo, 2015. Disponível em: <http://www.invistanoes.es.gov.br/images/contratos-de-competitividade/analisedos-setores/analise_moagem.pdf>. Acesso em: 19 mar. 2018.
MAPA MNISTÉRIO DA AGRICULTURA, ABASTECIMENTO E PECUARIA. LEVANTAMENTO DE RECONHECIMENTO DOS SOLOS DO ESTADO DO RIO GRANDE DO SUL. Recife, 1973.
NOSOUHIAN, F.; MOSTOFINEJAD, D. BALAM, N. H.; MOSTOFINEJAD, D.; EFTEKHAR, M.
Use of carbonate precipitating bacteria to reduce water absorption of aggregates,Aci Materials Journal, v. 113, n. 3, May-June 2016. Disponivel em: <https://www.researchgate.net/profile/Nafise_Balam/publication/315177272_Use_of_carbonate_precipitating_bacteria_to_reduce_water_absorption_of_aggregates/links/59e700b3a6fdcc0e882d4649/Use-of-carbonate-precipitating-bacteria-to-reducewater-absorption-of-aggregates.pdf>. Acesso em: 18 mar. 2018. Doi: 10.14359/51688701.
RAMACHANDRAN, S. K.; RAMAKRISHNAN, V.; BANG, S. S. Remediation of Concrete Using Micro-Organisms. ACI Materials Journal, v. 98, n. 1, p. 99-234, January-February 2001. Disponivel em: <http://sphinx.murdoch.edu.au/units/extern/BIO301/teach/download/previous%20years/Chemostat/B.%20pasteuri%20literature%20examples/Concrete%20remediation%20with%20B.%20pasteurii.pdf>. Acesso em: 17 mar. 2018.
RAO, M. et al. Bioengineered Concrete - A Sustainable Self-Healing Construction Material. Research Journal of Engineering Sciences, Hyderabad, v. 2, n. 6, p. 45-51, June 2013. Disponível em: <https://www.researchgate.net/publication/284593385_Bioengineered_concreteA_sustainable_self-healing_construction_material>. Acesso em: 20 mar. 2018.
REDDY , V. S. et al. Strength Enhancement of Cement Mortar using Microorganisms -An Experimental Study. International Journal of Earth Sciences and Engineering, India, v. 04, n. 06, p. 933-936, October 2011. ISSN 0974-5904. Disponivel em: <https://www.researchgate.net/publication/268329038_Strength_Enhancement_of_Cement_Mortar_using_Microorganisms_-An_Experimental_Study >. Acesso em: 16 mar. 2018.
SIDDIQUE, R.; CHAHAL, N. K. Effect of ureolytic bacteria on concrete properties.
Construction and Building Materials, Patiala, v. 25, n. 10, p. 3791-3801, 11 April 2011. ISSN 0950-0618 Disponivel em: <http://www.sciencedirect.com/science/article/pii/S0950061811001504?via%3Dihub>. Acesso em: 17 mar. 2018. Doi: https://doi.org/10.1016/j.conbuildmat.2011.04.010.
VERMA, R. K. et al. Bio-Mineralization and Bacterial Carbonate Precipitation in Mortar and Concrete. Public Science Frameword, Roorkee, v. 1, n. 1, p. 5-11, 4 April 2015.
Disponivel em: <http://www.publicscienceframework.org/journal/allissues/bio.html>. Acesso em: 22 mar. 2018.
NEAPOLIS UNIVERSITY. Project Eco-Cement. 2012-2015. Disponível em:. Acesso em: 17 mar. 2018.
